SARS coronavirus 8b reduces viral replication by down-regulating E via an ubiquitin-independent proteasome pathway
Identifieur interne : 002463 ( Main/Exploration ); précédent : 002462; suivant : 002464SARS coronavirus 8b reduces viral replication by down-regulating E via an ubiquitin-independent proteasome pathway
Auteurs : Choong-Tat Keng [Singapour] ; Sara Kerström [Suède] ; Cynthia Sau-Wai Leung [République populaire de Chine] ; Leo L. M. Poon [République populaire de Chine] ; J. S. Malik Peiris [République populaire de Chine] ; Ali Mirazimi [Suède] ; Yee-Joo Tan [Singapour]Source :
- Microbes and Infection [ 1286-4579 ] ; 2010.
Descripteurs français
- KwdFr :
- Animaux, Cellules Vero, Infections à coronavirus (métabolisme), Mutagenèse dirigée, Proteasome endopeptidase complex (métabolisme), Protéines de l'enveloppe virale (métabolisme), Protéines virales (métabolisme), Protéines à fluorescence verte (génétique), Protéines à fluorescence verte (métabolisme), Régulation de l'expression des gènes viraux, Régulation négative, Réplication virale (génétique), Stabilité protéique, Techniques de knock-out de gènes, Ubiquitine (métabolisme), Ubiquitinylation (génétique), Virus du SRAS (génétique), Virus du SRAS (métabolisme), Virus du SRAS (physiologie).
- MESH :
- génétique : Protéines à fluorescence verte, Réplication virale, Ubiquitinylation, Virus du SRAS.
- métabolisme : Infections à coronavirus, Proteasome endopeptidase complex, Protéines de l'enveloppe virale, Protéines virales, Protéines à fluorescence verte, Ubiquitine, Virus du SRAS.
- physiologie : Virus du SRAS.
- Animaux, Cellules Vero, Mutagenèse dirigée, Régulation de l'expression des gènes viraux, Régulation négative, Stabilité protéique, Techniques de knock-out de gènes.
English descriptors
- KwdEn :
- Animals, Chlorocebus aethiops, Coronavirus Infections (metabolism), Down-Regulation, Gene Expression Regulation, Viral, Gene Knockout Techniques, Green Fluorescent Proteins (genetics), Green Fluorescent Proteins (metabolism), Mutagenesis, Site-Directed, Proteasome Endopeptidase Complex (metabolism), Protein Stability, SARS Virus (genetics), SARS Virus (metabolism), SARS Virus (physiology), Ubiquitin (metabolism), Ubiquitination (genetics), Vero Cells, Viral Envelope Proteins (metabolism), Viral Proteins (metabolism), Virus Replication (genetics).
- MESH :
- chemical , genetics : Green Fluorescent Proteins.
- genetics : SARS Virus, Ubiquitination, Virus Replication.
- metabolism : Coronavirus Infections, Green Fluorescent Proteins, Proteasome Endopeptidase Complex, SARS Virus, Ubiquitin, Viral Envelope Proteins, Viral Proteins.
- physiology : SARS Virus.
- Animals, Chlorocebus aethiops, Down-Regulation, Gene Expression Regulation, Viral, Gene Knockout Techniques, Mutagenesis, Site-Directed, Protein Stability, Vero Cells.
Abstract
The severe acute respiratory syndrome coronavirus (SARS-CoV) 8b protein, which is not expressed by other known coronaviruses, can down-regulate the envelope (E) protein via a proteasome-dependent pathway. Here, we showed that the down-regulation of E is not dependent on the lysine residues on 8b and the reduction of polyubiquitination of E mutants is not correlated with their down-regulation by 8b, suggesting an ubiquitin-independent proteasome pathway is involved. A time-course study revealed that 8b was expressed at late-stages of SARS-CoV infection. By using Vero E6 cells stably expressing green fluorescence protein-tagged 8b, ectopic expression of 8b was shown to significantly reduce the production of progeny virus and down-regulate E expression. Taken together, these results suggest that 8b negatively modulates virus replication by down-regulating E via an ubiquitin-independent proteasome pathway.
Url:
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7110893
- https://hal-riip.archives-ouvertes.fr/pasteur-00588905
DOI: 10.1016/j.micinf.2010.10.017
PubMed: 21035562
PubMed Central: 7110893
Affiliations:
- République populaire de Chine, Singapour, Suède
- Svealand
- Stockholm
- Université nationale de Singapour
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Le document en format XML
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Microbiological Preparedness, Swedish Institute for Infectious Disease Control, SE-171 82 Solna, Sweden</nlm:aff><country xml:lang="fr">Suède</country><wicri:regionArea>Center for Microbiological Preparedness, Swedish Institute for Infectious Disease Control, SE-171 82 Solna</wicri:regionArea><wicri:noRegion>SE-171 82 Solna</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:aff id="affc">Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm, Sweden</nlm:aff><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm</wicri:regionArea><placeName><settlement type="city">Stockholm</settlement><region nuts="2">Svealand</region></placeName></affiliation></author><author><name sortKey="Leung, Cynthia Sau Wai" sort="Leung, Cynthia Sau Wai" uniqKey="Leung C" first="Cynthia Sau-Wai" last="Leung">Cynthia Sau-Wai Leung</name><affiliation wicri:level="1"><nlm:aff id="affd">Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Poon, Leo L M" sort="Poon, Leo L M" uniqKey="Poon L" first="Leo L. M." last="Poon">Leo L. M. Poon</name><affiliation wicri:level="1"><nlm:aff id="affd">Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Peiris, J S Malik" sort="Peiris, J S Malik" uniqKey="Peiris J" first="J. S. Malik" last="Peiris">J. S. Malik Peiris</name><affiliation wicri:level="1"><nlm:aff id="affd">Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="affe">HKU-Pasteur Research Centre, Hong Kong Special Administrative Region, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>HKU-Pasteur Research Centre, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Mirazimi, Ali" sort="Mirazimi, Ali" uniqKey="Mirazimi A" first="Ali" last="Mirazimi">Ali Mirazimi</name><affiliation wicri:level="1"><nlm:aff id="affb">Center for Microbiological Preparedness, Swedish Institute for Infectious Disease Control, SE-171 82 Solna, Sweden</nlm:aff><country xml:lang="fr">Suède</country><wicri:regionArea>Center for Microbiological Preparedness, Swedish Institute for Infectious Disease Control, SE-171 82 Solna</wicri:regionArea><wicri:noRegion>SE-171 82 Solna</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:aff id="affc">Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm, Sweden</nlm:aff><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm</wicri:regionArea><placeName><settlement type="city">Stockholm</settlement><region nuts="2">Svealand</region></placeName></affiliation></author><author><name sortKey="Tan, Yee Joo" sort="Tan, Yee Joo" uniqKey="Tan Y" first="Yee-Joo" last="Tan">Yee-Joo Tan</name><affiliation wicri:level="1"><nlm:aff id="affa">Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology, Singapore</nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology</wicri:regionArea><wicri:noRegion>Institute of Molecular and Cell Biology</wicri:noRegion></affiliation><affiliation wicri:level="4"><nlm:aff id="afff">Department of Microbiology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore, Singapore</nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Department of Microbiology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore</wicri:regionArea><orgName type="university">Université nationale de Singapour</orgName></affiliation></author></analytic><series><title level="j">Microbes and Infection</title><idno type="ISSN">1286-4579</idno><idno type="eISSN">1769-714X</idno><imprint><date when="2010">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Coronavirus Infections (metabolism)</term><term>Down-Regulation</term><term>Gene Expression Regulation, Viral</term><term>Gene Knockout Techniques</term><term>Green Fluorescent Proteins (genetics)</term><term>Green Fluorescent Proteins (metabolism)</term><term>Mutagenesis, Site-Directed</term><term>Proteasome Endopeptidase Complex (metabolism)</term><term>Protein Stability</term><term>SARS Virus (genetics)</term><term>SARS Virus (metabolism)</term><term>SARS Virus (physiology)</term><term>Ubiquitin (metabolism)</term><term>Ubiquitination (genetics)</term><term>Vero Cells</term><term>Viral Envelope Proteins (metabolism)</term><term>Viral Proteins (metabolism)</term><term>Virus Replication (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Infections à coronavirus (métabolisme)</term><term>Mutagenèse dirigée</term><term>Proteasome endopeptidase complex (métabolisme)</term><term>Protéines de l'enveloppe virale (métabolisme)</term><term>Protéines virales (métabolisme)</term><term>Protéines à fluorescence verte (génétique)</term><term>Protéines à fluorescence verte (métabolisme)</term><term>Régulation de l'expression des gènes viraux</term><term>Régulation négative</term><term>Réplication virale (génétique)</term><term>Stabilité protéique</term><term>Techniques de knock-out de gènes</term><term>Ubiquitine (métabolisme)</term><term>Ubiquitinylation (génétique)</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (métabolisme)</term><term>Virus du SRAS (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Green Fluorescent Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term><term>Ubiquitination</term><term>Virus Replication</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines à fluorescence verte</term><term>Réplication virale</term><term>Ubiquitinylation</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Coronavirus Infections</term><term>Green Fluorescent Proteins</term><term>Proteasome Endopeptidase Complex</term><term>SARS Virus</term><term>Ubiquitin</term><term>Viral Envelope Proteins</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Infections à coronavirus</term><term>Proteasome endopeptidase complex</term><term>Protéines de l'enveloppe virale</term><term>Protéines virales</term><term>Protéines à fluorescence verte</term><term>Ubiquitine</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Down-Regulation</term><term>Gene Expression Regulation, Viral</term><term>Gene Knockout Techniques</term><term>Mutagenesis, Site-Directed</term><term>Protein Stability</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Mutagenèse dirigée</term><term>Régulation de l'expression des gènes viraux</term><term>Régulation négative</term><term>Stabilité protéique</term><term>Techniques de knock-out de gènes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The severe acute respiratory syndrome coronavirus (SARS-CoV) 8b protein, which is not expressed by other known coronaviruses, can down-regulate the envelope (E) protein via a proteasome-dependent pathway. Here, we showed that the down-regulation of E is not dependent on the lysine residues on 8b and the reduction of polyubiquitination of E mutants is not correlated with their down-regulation by 8b, suggesting an ubiquitin-independent proteasome pathway is involved. A time-course study revealed that 8b was expressed at late-stages of SARS-CoV infection. By using Vero E6 cells stably expressing green fluorescence protein-tagged 8b, ectopic expression of 8b was shown to significantly reduce the production of progeny virus and down-regulate E expression. Taken together, these results suggest that 8b negatively modulates virus replication by down-regulating E via an ubiquitin-independent proteasome pathway.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Peiris, J S" uniqKey="Peiris J">J.S. Peiris</name></author><author><name sortKey="Lai, S T" uniqKey="Lai S">S.T. 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Sniegowski</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>République populaire de Chine</li><li>Singapour</li><li>Suède</li></country><region><li>Svealand</li></region><settlement><li>Stockholm</li></settlement><orgName><li>Université nationale de Singapour</li></orgName></list><tree><country name="Singapour"><noRegion><name sortKey="Keng, Choong Tat" sort="Keng, Choong Tat" uniqKey="Keng C" first="Choong-Tat" last="Keng">Choong-Tat Keng</name></noRegion><name sortKey="Tan, Yee Joo" sort="Tan, Yee Joo" uniqKey="Tan Y" first="Yee-Joo" last="Tan">Yee-Joo Tan</name><name sortKey="Tan, Yee Joo" sort="Tan, Yee Joo" uniqKey="Tan Y" first="Yee-Joo" last="Tan">Yee-Joo Tan</name></country><country name="Suède"><noRegion><name sortKey=" Kerstrom, Sara" sort=" Kerstrom, Sara" uniqKey=" Kerstrom S" first="Sara" last=" Kerström">Sara Kerström</name></noRegion><name sortKey=" Kerstrom, Sara" sort=" Kerstrom, Sara" uniqKey=" Kerstrom S" first="Sara" last=" Kerström">Sara Kerström</name><name sortKey="Mirazimi, Ali" sort="Mirazimi, Ali" uniqKey="Mirazimi A" first="Ali" last="Mirazimi">Ali Mirazimi</name><name sortKey="Mirazimi, Ali" sort="Mirazimi, Ali" uniqKey="Mirazimi A" first="Ali" last="Mirazimi">Ali Mirazimi</name></country><country name="République populaire de Chine"><noRegion><name sortKey="Leung, Cynthia Sau Wai" sort="Leung, Cynthia Sau Wai" uniqKey="Leung C" first="Cynthia Sau-Wai" last="Leung">Cynthia Sau-Wai Leung</name></noRegion><name sortKey="Peiris, J S Malik" sort="Peiris, J S Malik" uniqKey="Peiris J" first="J. S. Malik" last="Peiris">J. S. Malik Peiris</name><name sortKey="Peiris, J S Malik" sort="Peiris, J S Malik" uniqKey="Peiris J" first="J. S. Malik" last="Peiris">J. S. Malik Peiris</name><name sortKey="Poon, Leo L M" sort="Poon, Leo L M" uniqKey="Poon L" first="Leo L. M." last="Poon">Leo L. M. Poon</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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